Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, Gram-negative coccobacillus. It grows under aerobic conditions, is catalase positive and oxidase negative. A. calcoaceticus is a part of the A. calcoaceticus-A. baumannii complex together with Acinetobacter baumannii, Acinetobacter nosocomialis, Acinetobacter pitti and Acinetobacter seifertii.
A. calcoaceticus can be used as an alternative to A. baumannii in the laboratory setting. The interchangeability of the two species stems from their extreme degree of similarity and ability to form the A. calcoaceticus-A. baumannii complex. Other contributing factors include the cost-effective nature of A. calcoaceticus compared to A. baumannii and the ability of A. calcoaceticus to readily uptake DNA.